Schlagwörter
Skills
- Thomas Mohr participated in long standing cooperations with various pharmaceutical companies and institutes (e.g. Institute of Cancer Research, Medical University of Vienna, Marlyn Neutraceuticals, Phoenix, Arizona, USA, Volopharm, Wels, Austria) that resulted in a number of papers and publications.
- Cell isolation and culture techniques (implementation as well as practice)
- Isolation of endothelial cells, epithelial cells, fibroblasts, peripheral blood monocytes
- 3D culture (matrigel embedding)
- High density culture (hollow fiber reactor)
- co-culture techniques
- protein-free cell culture
- Establishment of marker panels for cells for FACS and Immunocytochemistry
- ELISA techniques (implementation as well as practice)
- Sandwich ELISA, Fixed Cell ELISA
- PCR-Techniques
- Immunocytochemical methods (fluorescence and visible)
- Confocal microscopy
- Functional assays (cell migration, formation of 3D structures, matrigel-based cell invasion assays)
- Statistical design and analysis of experiments
- Profound knowledge of relevant analysis and statistical software (SPSS, GraphPad Prism, R, Bioconductor, FlowJo)
- Profound knowledge of -omics analysis methods (microarray, RNASeq and proteomics) with a focus on network and pathway analysis methods.
- Numerous lectures about methods in putting -omics data into biological context at the Medical University of Vienna and the Ludwig Boltzmann Institut for Cancer Research, Vienna.
Projekthistorie
Reisebereitschaft
Verfügbar in den Ländern
Österreich